Induced Pluripotent Stem Cells PCR Array 誘導多能干細胞PCR芯片

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Induced Pluripotent Stem Cells PCR Array 誘導多能干細胞PCR芯片

誘導多能干細胞PCR芯片用于研究參與誘導多能干細胞(iPSC)的84個關(guān)鍵基因的表達。iPSCs有望治療大量疾病，通過轉(zhuǎn)化成年體細胞為多能細胞，使其分化成多種細胞類型中的任何一個，胚胎干細胞使用的倫理性。這個流程首先使轉(zhuǎn)染或轉(zhuǎn)導體細胞(如角化細胞)，構(gòu)建異位表達特定的轉(zhuǎn)錄因子的復合體(KLF4,MYC POU5F1,和/或SOX2)。這些轉(zhuǎn)錄因子重組或誘導體細胞“去分化”,失去原始細胞類型的標記并獲得多能細胞的標記。
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簡介：Induced Pluripotent Stem Cells PCR Array 誘導多能干細胞PCR芯片
提供商：SAbiosciences
服務(wù)名稱：誘導多能干細胞PCR芯片
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Induced Pluripotent Stem Cells PCR Array

誘導多能干細胞PCR芯片
 
ProductSpeciesTechnologyCat. No.
Induced Pluripotent Stem Cells PCR ArrayHumanGene ExpressionPAHS-092Z
Induced Pluripotent Stem Cells PCR ArrayMouseGene ExpressionPAMM-092Z
Induced Pluripotent Stem Cells PCR ArrayRatGene ExpressionPARN-092Z
he Human Induced Pluripotent Stem Cells RT2 Profiler PCR Array profiles the expression of 84 key genes involved in induced pluripotent stem cell (iPSC) research. iPSCs hold the promise to provide treatments for a multitude of diseases by converting adult somatic cells into pluripotent cells that are able to differentiate into any one of a variety of cell types, avoiding the ethics of embryonic stem cell (ESC) use. The process starts by transfecting or transducing somatic cells (such as keratinocytes) with constructs ectopically expressing a combination of specific transcription factors (KLF4, MYC, POU5F1, and/or SOX2). These transcription factors reprogram or induce the somatic cells to "dedifferentiate", losing markers of the original cell type and gaining markers of pluripotent cells. Often, a combination of additional transcription factors (such as ESRRB, LIN28A, NANOG, MYCN, and/or NR5A2) increases induction efficiency. To control the procedure, the expression of multiple gene classes included on this array must be monitored simultaneously: representative parental cell line genes, the ectopically expressed transcription factors, markers of iPSCs, and markers of the redifferentiation into ectoderm, endoderm, and mesoderm. ESCs and iPSCs have proven to not be functionally identical. Therefore, this array also analyzes genes highly expressed in both cell types to help distinguish them and better understand their differences. Because the expression of typical housekeeping or reference genes often proves inconsistent in these types of studies, the array includes another gene (NAT1) used in iPSC gene expression for data normalization if needed. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in the induced pluripotent stem cell dedifferentiation and redifferentiation processes with this array.
The RT2 Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available.
 
誘導多能干細胞PCR芯片用于研究參與誘導多能干細胞(iPSC)的84個關(guān)鍵基因的表達。iPSCs有望治療大量疾病，通過轉(zhuǎn)化成年體細胞為多能細胞，使其分化成多種細胞類型中的任何一個，胚胎干細胞使用的倫理性。這個流程首先使轉(zhuǎn)染或轉(zhuǎn)導體細胞(如角化細胞)，構(gòu)建異位表達特定的轉(zhuǎn)錄因子的復合體(KLF4,MYC POU5F1,和/或SOX2)。這些轉(zhuǎn)錄因子重組或誘導體細胞“去分化”,失去原始細胞類型的標記并獲得多能細胞的標記。通常，額外的轉(zhuǎn)錄因子的結(jié)合(如ESRRB LIN28A,NANOG MYCN,和/或NR5A2)會增加感應(yīng)效率。為了控制該程序, 該芯片上的多種基因類群的表達必須同時被檢測:代表親本的細胞系基因,異位表達的轉(zhuǎn)錄因子,iPSCs的標記,再分化成外胚層、內(nèi)胚層和中胚層的標記。ESCs和iPSCs已被證明功能不完全相同。因此,這個芯片分析兩者細胞類型都高表達的基因來幫助區(qū)分和更好的理解他們之間的差異。因為典型的管家或參考基因的表達往往在這些類型的研究證明不一致,該芯片包含另一個基因(NAT1) 用于iPSC基因表達數(shù)據(jù)標準化。每張芯片含一個對照組使得分析數(shù)據(jù)時可以用相對定量ΔΔCT方法評估逆轉(zhuǎn)錄效率,基因組DNA污染,和PCR效率。利用這張芯片，通過實時定量PCR，可以簡易且可靠地分析誘導多能干細胞去分化和再分化過程關(guān)鍵基因的表達。
 
PCR芯片僅用于分子生物學應(yīng)用。本產(chǎn)品不用于疾病的診斷、預(yù)防和治療。
提供96孔板——384 -(4 x 96)板,和100孔板
 
Somatic Cell Markers:
Keratinocyte: CDH1, GJB2, KRT15, NUMB.
Blood Stem Cell: CD34.
Fibroblast: COL1A1, NCAM1.
Neuronal Stem Cell: EMX2, FABP7, NES, OLIG2.
Cardiomyocyte: PECAM1.
Germ Cells: SYCP3.
Induced Pluripotent Stem Cells:
iPSC Markers: DNMT3B, PODXL, ZFP42.
Other iPSC-Related Genes: ACTC1, ALDH1A1, ALDH2, APC, BGLAP, BMP2, BRIX1 (BXDC2), CCNE1, CD9, CDH2, COL2A1, COL9A1, EP300, FGF4, FGFR1, FOXD3, GABRB3, GJA1, GRB7, HDAC2, KAT2A, KAT7, KAT8, LEFTY1, LEFTY2, NODAL, PARD6A, REST, RUNX2, TERT.
Reprogramming Factors: KLF4, MYC, POU5F1 (Oct3/4), SOX2.
Other Reprogramming Factors: ESRRB, LIN28A, NANOG, MYCN, NR5A2.
Reprogramming Enhancers & Inhibitors: AICDA, TP53, UTF1.
Embryonic Stem Cells:
ESC Markers: ALPL, GDF3, TDGF1 (CRIPTO), TERT.
Other ESC-Related Genes: CCNA2, CDK1 (CDC2), CDC42, DPPA2, DPPA3, FGF2, HSPA9, MYBL2, OTX2, SOX15, TBX3, TCF3.
Pluripotency Markers: ALPL, DNMT3B, FGF4, FOXD3, GDF3, LEFTY1, LEFTY2, NODAL, PODXL, UTF1, ZFP42.
Early Differentiation Markers:
Ectoderm: COL1A1, NCAM1, NES, PAX6, TUBB3.
Mesoderm: CD34, GATA2, HAND1, MESP1, PECAM1, RUNX1.
Endoderm: FOXA2, GATA4, HNF4A, SOX17.
Housekeeping Gene: NAT1.


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