地區(qū): | 上海 |
簡介: | 上海 |
提供商: | SABio |
服務(wù)名稱: | PCR芯片 |
EGF/PDGF信號(hào)通路PCR芯片可用于研究與EGF/PDGF信號(hào)通路轉(zhuǎn)導(dǎo)有關(guān)的84個(gè)基因的表達(dá)。胚胎干細(xì)胞基因芯片可用于研究參與維護(hù)胚胎干細(xì)胞多能性和自我更新狀態(tài)的84個(gè)管家基因的表達(dá)。胚胎干細(xì)胞(ESC)在治療疾病具有巨大潛力。然而,把他們變成一個(gè)安全的治療因素仍然需要深入了解細(xì)胞譜系的保證和分化的控制機(jī)制。該芯片包含的基因包括胚胎干細(xì)胞細(xì)胞特定的基因,維持多能性和自我更新特點(diǎn)和對(duì)于誘導(dǎo)多能干細(xì)胞(iPSC)分化必須的基因。該芯片還包括分化標(biāo)記物,可用于監(jiān)測ESC分化的早期事件。每個(gè)芯片的數(shù)據(jù)分析使用ΔΔCT方法進(jìn)行相對(duì)量化,并包括多個(gè)質(zhì)控如逆轉(zhuǎn)錄性能、基因組DNA污染、PCR性能。通過實(shí)時(shí)定量PCR的方法,研究者即能夠利用該芯片簡單可靠地同時(shí)檢測與胚胎干細(xì)胞維持和分化有關(guān)的基因表達(dá)。 Embryonic Stem Cell (ESC) Specific Genes: Markers: ALPL, GDF3 (VGR2), TDGF1, TERT. Chromatin Regulators: CHD1, CHD7, KAT5. Cofactors: CNOT3, NR0B1, PAF1, TRIM28. Transcription Factors: ESRRB, KLF4, LIN28A, MYC, NANOG, POU5F1 (OCT4), PRDM14, SALL4, SMAD1, SMAD2, SMAD3, SOX2, STAT3, TBX3, TCF3, THAP11, UTF1, ZFX. Pluripotency: AICDA, CCNA2, CDC42, CDK1 (CDC2), DPPA2, DPPA3, DPPA4, DPPA5, FGF2 (bFGF), HSPA9, MYBL2, NR5A2, NR6A1, OTX2, RIF1, SCN1A, SOX15, TCL1A, ZFP42. iPSC Genes/Enhancers: AICDA, KLF4, LIN28A, MYC, NANOG, POU5F1 (OCT4), SOX2, UTF1. Germ Layers: Ectoderm: EN2, FGF5, MEIS1, NCAM1, PAX6. Mesoderm: DES, HAND1, MESP1, MIXL1, MYOD1, T (Brachyury). Endoderm: GATA1, GATA6, GSC, HNF4A, SOX17, SOX7. Early Cell Types: Neural Progenitors: GFAP, NES, OLIG2, SOX3. Cardiac Progenitors: ISL1, NKX2-5. Early Smooth Muscle Cells: ACTA2, TAGLN. Hematopoietic Stem Cells/Early Endothelial Cells: ETV2, FLII, GATA2, TAL1, TEK (TIE2). Mesenchymal Stem Cells: CD44, ENG (EVI1), NT5E, THY1. Hepatic Tissue: ALB, TAT. |