癌癥干細胞miRNA PCR芯片 Cancer Stem Cells miScript miRNA PCR Array

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癌癥干細胞miRNA PCR芯片 Cancer Stem Cells miScript miRNA PCR Array

癌癥干細胞miRNA PCR芯片 Cancer Stem Cells miScript miRNA PCR Array
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Cancer Stem Cells miScript miRNA PCR Array

癌癥干細胞miRNA PCR芯片

 
ProductSpeciesTechnologyCat. No.
Cancer Stem Cells miScript miRNA PCR ArrayHumanmiRNA ExpressionMIHS-118Z
Cancer Stem Cells miScript miRNA PCR ArrayMousemiRNA ExpressionMIMM-118Z
Cancer Stem Cells miScript miRNA PCR ArrayRatmiRNA ExpressionMIRN-118Z
The Human Cancer Stem Cells miScript miRNA PCR Array profiles the expression of 84 miRNAs involved in cancer stem cell (CSC) biology. Cancer researchers have struggled with the vexing problem that although many cancer drugs dramatically reduce the size of the tumors, most cancers eventually relapse. Dynamic changes in cancer cell populations during treatment suggest that a small population of cells resistant to current therapies is ultimately responsible for the re-growth of tumors. Furthermore, studies imply that these cells may provide a reservoir for the generation and propagation of mutant cells, providing further resistance to therapy. The cancer stem cell hypothesis posits that only a very rare population of cells within tumors has the capacity for limitless self-renewal. This concept has important therapeutic implications, and may explain why many cancers return even after treatment removes any detectable tumor cells. If current treatments do not eliminate CSCs, then they may regenerate the tumor once treatment stops. Advances in technology have allowed the prospective identification and purification of CSCs from various different types of cancers for further characterization. Recent studies support the critical regulatory roles of the miRNAs represented by this array in CSC pluripotency, self-renewal, differentiation, metastasis, and tumor suppression. Profiling the differential expression of miRNAs in CSCs would allow a better understanding of miRNA function in CSCs and may provide invaluable insights into the molecular mechanisms of tumorigenesis and tumor progression. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of cancer stem cell miRNAs can be easily and reliably analyzed with this miScript miRNA PCR Array.
96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available
 
癌癥干細胞miRNA PCR芯片用于研究與腫瘤干細胞(CSCs)生物學相關的84個miRNA的表達。癌癥研究人員疲于應對棘手的問題,盡管許多抗癌藥物大幅減少腫瘤的大小,最終大多數(shù)癌癥復發(fā)。在治療期間癌癥細胞種群的動態(tài)變化表明,一個小種群細胞抵抗目前的治療方法是最終負責腫瘤復發(fā)。此外,研究表明,這些細胞可能會提供一個突變細胞的生成和傳播儲蓄池提供進一步的抵抗治療。腫瘤干細胞假說認為在腫瘤中只有很少的細胞具有無限的自我更新能力。這一概念具有重要的治療意義,甚至也許可以解釋為什么許多癌癥恢復甚至在治療清除任何檢測到的腫瘤細胞后。目前的治療如果不消除癌癥干細胞,那么一旦停止治療它們可能會再生腫瘤。最近技術的進步使得CSCs從不同類型的癌癥中做的預期鑒定和純化作進一步鑒定。這個芯片包括CSC分子標記和調節(jié)CSC增殖、自我更新和多潛能幫助確保CSC隔離培養(yǎng)的穩(wěn)定性的基因。還包括參與CSC不對稱細胞分裂、遷移和轉移、促進CSC鑒定的相關信號轉導通路的基因，以及當前已測試的治療靶點。分析CSCs中miRNA的差異表達將助于更好地理解腫瘤發(fā)生和腫瘤進展的分子機制。每張芯片含一個對照組使得分析數(shù)據(jù)時可以用相對定量ΔΔCT方法評估逆轉錄效率,基因組DNA污染,和PCR效率。利用這張芯片，通過實時定量PCR，可以簡易且可靠地分析癌癥干細胞miRNA的表達。
Pluripotency & Differentiation: let-7a-5p, miR-122-5p, miR-122-5p, miR-134-5p, miR-137, miR-145-5p, miR-181a-5p, miR-181c-5p, miR-183-5p, miR-184, miR-200a-3p, miR-200c-3p, miR-320d, miR-34a-5p.
Self-Renewal & Proliferation: miR-134-5p, miR-137, miR-141-3p, miR-182-5p, miR-183-5p, miR-184, miR-200b-3p, miR-203a, miR-21-5p, miR-296-5p, miR-302a-3p, miR-429, miR-9-5p, miR-96-5p.
Migration & Metastasis: let-7a-5p, let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-16-5p, miR-17-5p, miR-181b-5p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-22-3p, miR-302a-3p, miR-373-3p, miR-517a-3p, miR-9-5p.
Oncogenesis & Tumor Suppression: let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-181b-5p, miR-373-3p, miR-517a-3p.
Differentially Expressed miRNAs:
Up-Regulated: miR-105-5p, miR-106b-5p, miR-125b-5p, miR-132-3p, miR-135b-5p, miR-142-3p, miR-146a-5p, miR-146b-5p, miR-150-5p, miR-155-5p, miR-16-2-3p, miR-193a-3p, miR-199b-5p, miR-20a-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-25-3p, miR-299-5p, miR-29b-3p, miR-31-5p, miR-409-3p, miR-423-5p, miR-455-3p, miR-455-5p, miR-494-3p, miR-516a-5p, miR-522-3p, miR-744-5p.
Down-Regulated: miR-103a-3p, miR-10a-5p, miR-1181, miR-1207-5p, miR-128-3p, miR-130a-3p, miR-15a-5p, miR-15b-5p, miR-185-5p, miR-425-5p, miR-451a, miR-486-5p, miR-548d-5p, miR-636.
Regulated: miR-107, miR-142-5p, miR-151a-3p, miR-365a-3p.



 

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template 


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