地區(qū): | 上海 |
簡(jiǎn)介: | 上海 |
提供商: | SABio |
服務(wù)名稱: | PCR芯片 |
細(xì)胞外基質(zhì)&粘附因子PCR芯片可用于研究對(duì)細(xì)胞-細(xì)胞和細(xì)胞-基質(zhì)相互作用的84個(gè)基因的表達(dá)。細(xì)胞外基質(zhì)(ECM)中附著蛋白多糖和糖蛋白(如纖連蛋白,層粘連蛋白和膠原蛋白),通過(guò)細(xì)胞表面的粘附分子來(lái)定義組織的形狀,結(jié)構(gòu)和功能,形成和打斷細(xì)胞與其他細(xì)胞的聯(lián)系;此外,ECM在正常過(guò)程如細(xì)胞生長(zhǎng),分裂,分化和遷移的接觸中發(fā)揮重要作用。疾病狀態(tài)(轉(zhuǎn)移,類風(fēng)濕關(guān)節(jié)炎,心血管疾病和中樞神經(jīng)系統(tǒng)紊亂)和病理生理過(guò)程(傷口愈合和炎癥)也參與了ECM重構(gòu)和在細(xì)胞表面上表達(dá)的細(xì)胞粘附分子類的變化。該芯片包含ECM蛋白,包括基底膜成分,膠原蛋白,基因和其他基因定義的ECM結(jié)構(gòu);基質(zhì)和重構(gòu)ECM的其他金屬蛋白酶,及其抑制劑;整合素,選擇素,細(xì)胞粘附分子家族成員(ICAM,ECAM,NCAM,PECAM,和VCAM),其他對(duì)細(xì)胞粘附和細(xì)胞骨架連接起重要作用的基因,如連環(huán)蛋白。通過(guò)實(shí)時(shí)定量PCR的方法,研究者即能夠利用該芯片簡(jiǎn)單可靠地同時(shí)檢測(cè)與細(xì)胞粘附和ECM相關(guān)的基因表達(dá)。 Cell Adhesion Molecules: Transmembrane Molecules: CD44, CDH1, HAS1, ICAM1, ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGA7, ITGA8, ITGAL, ITGAM, ITGAV, ITGB1, ITGB2, ITGB3, ITGB4, ITGB5, MMP14, MMP15, MMP16, NCAM1, PECAM1, SELE, SELL, SELP, SGCE, SPG7, VCAM1. Cell-Cell Adhesion: CD44, CDH1, COL11A1, COL14A1, COL6A2, CTNND1, ICAM1, ITGA8, VCAM1. Cell-Matrix Adhesion: ADAMTS13, CD44, ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGA7, ITGA8, ITGAL, ITGAM, ITGAV, ITGB1, ITGB2, ITGB3, ITGB4, ITGB5, SGCE, SPP1, THBS3. Other Adhesion Molecules: CNTN1, COL12A1, COL15A1, COL16A1, COL5A1, COL6A1, COL7A1, COL8A1, VCAN, CTGF, CTNNA1, CTNNB1, CTNND2, FN1, KAL1, LAMA1, LAMA2, LAMA3, LAMB1, LAMB3, LAMC1, THBS1, THBS2, CLEC3B, TNC, VTN. Extracellular Matrix Proteins: Basement Membrane Constituents: COL4A2, COL7A1, LAMA1, LAMA2, LAMA3, LAMB1, LAMB3, LAMC1, SPARC. Collagens & ECM Structural Constituents: COL11A1, COL12A1, COL14A1, COL15A1, COL16A1, COL1A1, COL4A2, COL5A1, COL6A1, COL6A2, COL7A1, COL8A1, FN1, KAL1. ECM Proteases: ADAMTS1, ADAMTS13, ADAMTS8, MMP1, MMP10, MMP11, MMP12, MMP13, MMP14, MMP15, MMP16, MMP2, MMP3, MMP7, MMP8, MMP9, SPG7, TIMP1. ECM ProteaseInhibitors: COL7A1, KAL1, THBS1, TIMP1, TIMP2, TIMP3. Other ECM Molecules: VCAN, CTGF, ECM1, HAS1, SPP1, TGFBI, THBS2, THBS3, CLEC3B, TNC, VTN. |