心臟毒性PCR芯片可用于研究由藥物或化合物誘導(dǎo)而發(fā)生心臟損傷的84個(gè)關(guān)鍵基因的表達(dá)。毒性反應(yīng)是藥物上市的檢測指標(biāo)之一,其中就包括心臟毒性反應(yīng)的檢測。出于安全考慮,在過去40年,約有10%的藥品因?yàn)閷?duì)心血管有影響而撤出臨床市場。傳統(tǒng)的形態(tài)學(xué)研究昂貴又耗時(shí),且很難分辨是否為藥物或化合物引發(fā)的心臟毒性反應(yīng)。這一芯片包含了多個(gè)模型中由于藥物或化合物引起的心臟損傷的生物標(biāo)志物基因,既減少了實(shí)驗(yàn)周期和成本,也能在研究最初階段就發(fā)現(xiàn)和剔除會(huì)引起心臟毒性反應(yīng)的藥物。通過實(shí)時(shí)定量PCR的方法,研究者即能夠利用該芯片簡單可靠地同時(shí)檢測心臟毒性相關(guān)基因的表達(dá)。 Up-Regulated: Muscle Contraction: KBTBD10. Angiogenesis: IL6, PLAU, SERPINE1, TGFB2. Signal Transduction: ABRA, ADRA2A, CCR1, CREM, IL6, RND1, SIK1, TGFB2, TIAM1. Cell Cycle: BCAT1, FOSL1, HSPA2, SIK1, TGFB2. Development: BTG2, CREM, EGR1, FHL1, HSPA2, IL6, RND1, SIK1, SPP1, TGFB2, TIAM1, TIMP1, UCP1, VCAN. Metabolism (Regulation & Mechanism): ABRA, ADRA2A, BTG2, CH25H, CREM, EGR1, FOSL1, IL6, PDK4, PLA2G4A, PUM2, RBM3, SIK1, TCF4, TGFB2, TIMP1. Migration / Motility: ABHD2, CCL7, CCR1, FOSL1, IL6, PLAU, PPBP, PVR, TGFB2. Other: CD14, CFD, CKM, FCGR2B, GPM6A, HAMP, KBTBD5, MT1F, PLUNC, REG3G, TUBB6, UCK2. Down-Regulated: Muscle Contraction: ACTA1, GJA1, KCNJ12, RPS6KB1. Angiogenesis: COL15A1, POSTN, VEGFA. Signal Transduction: AIFM1, DUSP8, GJA1, IGFBP5, ITPR2, RPS6KB1, S1PR2, THRAP3. Cell Cycle: CSNK2A2, MCM6, PSMA2, PSMD7, TXNIP. Development: ACTA1, COL15A1, COL3A1, GJA1, IGFBP5, PLN, RPS6KB1, SOX4, TXNIP. Metabolism (Regulation & Mechanism): ASH1L, ATP5J, DUSP8, IGFBP5, MCM6, NFIB, PPP1R14C, PRKAB2, S1PR2, SOX4, THRAP3, TXNIP, ZNF148, ZNF23. Migration / Motility: IGFBP5, NEXN, RPS6KB1. Other: AK3, BGN, BSN, HSPH1, IFT20, PKN2, SLC4A3, UBA5, UBXN2A, VIM, WIPI1. |