纖維化miRNAPCR芯片 Fibrosis miScript miRNA PCR Array

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Fibrosis miScript miRNA PCR Array

纖維化miRNAPCR芯片

 
The Human Fibrosis miScript miRNA PCR Array profiles the expression of 84 miRNAs known to play a role in fibrosis. During tissue remodeling that occurs as part of wound healing, apoptosis removes myofibroblasts, and the deposited extracellular matrix (ECM) is remodeled to resemble the original tissue. Fibrosis occurs when inappropriate tissue remodeling results in excess ECM deposition due to inappropriate survival of myofibroblasts or lack of ECM proteolytic degradation. The inflammation and enhanced TGFβ signaling often present in fibrotic tissues causes cells to differentiate into myofibroblasts via epithelial-to-mesenchymal transition (EMT). miRNAs may play a role in conducting the pathogenesis of fibrosis by modulating the expression of genes involved in ECM remodeling and cell adhesion, inflammation, angiogenesis, TGFβ/CTGF signaling, and EMT. This array includes miRNAs that are differentially expressed during fibrosis and are known or predicted to modulate the expression of pro- and anti-fibrotic genes common to different fibrotic disorders such as systemic sclerosis, as well as liver, cardiac, kidney, and idiopathic pulmonary fibrosis. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of fibrosis. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs involved in fibrosis can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available
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纖維化miRNAPCR芯片用于研究在纖維化中發(fā)揮重要作用的84miRNA的表達(dá)。在組織重建期，肌成纖維細(xì)胞通過(guò)細(xì)胞凋亡被清除，沉積的細(xì)胞外基質(zhì)（ECM）被重塑，更像原始組織。若組織重塑異常，例如肌成纖維細(xì)胞異常生存或者ECM蛋白水解酶降解缺失，都有可能導(dǎo)致過(guò)量ECM沉積，則會(huì)造成纖維化。在纖維化的組織中，炎癥反應(yīng)和增強(qiáng)的TGF.信號(hào)通路，會(huì)導(dǎo)致細(xì)胞通過(guò)上皮間質(zhì)轉(zhuǎn)化（EMT）分化為肌成纖維細(xì)胞。與之相對(duì)的另一面，由于ECM降解被加強(qiáng)了，導(dǎo)致慢性損傷的組織重塑異常。芯片包括編碼ECM重建酶基因，TGF.信號(hào)通路分子和炎癥細(xì)胞因子，還包括其他與纖維化密切相關(guān)的基因。miRNA可能在進(jìn)行纖維化的發(fā)病機(jī)制中發(fā)揮作用,在ECM重塑和細(xì)胞粘附,炎癥,血管生成,TGFβ/ CTGF信號(hào),EMT,調(diào)節(jié)相關(guān)基因的表達(dá)。。這個(gè)芯片包含在纖維化過(guò)程差異表達(dá)的miRNA，以及已知或預(yù)測(cè)調(diào)節(jié)促進(jìn)和抑制纖維化基因的miRNA,這些基因常見(jiàn)于不同的纖維化疾病如系統(tǒng)性硬化癥,以及肝臟、心臟、腎臟、特發(fā)性肺纖維化。這個(gè)芯片的分析結(jié)果將助于了解纖維化發(fā)病的分子機(jī)制。每張芯片含一個(gè)對(duì)照組使得分析數(shù)據(jù)時(shí)可以用相對(duì)定量ΔΔCT方法評(píng)估逆轉(zhuǎn)錄效率,基因組DNA污染,和PCR效率。利用這張芯片，通過(guò)實(shí)時(shí)定量PCR，可以簡(jiǎn)易且可靠地分析纖維化相關(guān)miRNA的表達(dá)。
 
miScript miRNA PCR芯片僅用于分子生物學(xué)應(yīng)用。本產(chǎn)品不用于疾病的診斷、預(yù)防和治療。
提供96孔板——384 -(4 x 96)板,和100孔板
 
Pro-Fibrotic: miR-142-3p , miR-145-5p, miR-155-5p, miR-199b-5p, miR-21-5p, miR-215, miR-216a-5p, miR-27a-3p, miR-27b-3p, miR-32-5p, miR-338-5p, miR-34a-5p, miR-377-3p, miR-382-5p, miR-192-5p, miR-30a-5p, miR-208a, miR-5692a, miR-5011-5p.
Anti-Fibrotic: let-7d-5p, miR-107, miR-132-3p, miR-133a, miR-141-3p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-18a-5p, miR-194-5p, miR-19a-3p, miR-19b-3p, miR-200a-3p, miR-200b-3p, miR-204-5p, miR-20a-5p, miR-211-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-335-5p, miR-449a, miR-449b-5p, miR-590-5p, miR-92a-3p.
Extracellular Matrix Remodeling & Cell Adhesion: miR-199a-5p, miR-199b-5p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-449a, miR-21-5p, miR-204-5p, miR-25-3p, miR-7-5p, miR-196a-5p, miR-203a, miR-27b-3p, miR-27a-3p, miR-451a, miR-145-5p, miR-143-3p, miR-1, miR-10a-5p, miR-10b-5p, miR-661, miR-30a-5p, miR-16-5p, miR-217.
Inflammation: let-7d-5p, miR-204-5p, miR-122-5p, miR-146a-5p, miR-199a-5p, miR-155-5p, miR-21-5p, miR-129-5p, miR-142-3p, miR-503-5p.
Angiogenesis: let-7d-5p, miR-378a-3p, miR-372, miR-34a-5p, miR-29b-3p, miR-20a-5p, miR-200b-3p, miR-195-5p, miR-17-5p, miR-16-5p, miR-15b-5p, miR-150-5p, miR-145-5p, miR-126-3p, miR-107, miR-31-5p, miR-375.
Signal Transduction & Transcriptional Regulation: miR-26a-5p, miR-18a-5p, miR-122-5p, miR-133a, miR-20a-5p, miR-203a, miR-19a-3p, miR-17-5p, miR-146a-5p, miR-155-5p, miR-92a-3p, miR-29b-3p, miR-141-3p, miR-29a-3p, miR-204-5p, miR-590-5p, miR-372, miR-302b-3p, miR-19b-3p, miR-21-5p, miR-1, miR-217, miR-23a-3p, miR-328, miR-744-5p, miR-148a-3p, miR-451a, miR-125b-5p, miR-449a, miR-34a-5p, miR-29c-3p, miR-195-5p, miR-192-5p, miR-16-5p, miR-15b-5p, miR-663a, miR-30a-5p, miR-378a-3p, miR-145-5p, miR-146b-5p, miR-27a-3p, miR-324-5p, miR-324-3p, miR-223-3p, miR-491, miR-101-3p, miR-491-5p.
Epithelial-to-Mesenchymal Transition: let-7d-5p, miR-107, miR-155-5p, miR-199a-5p, miR-199b-5p, miR-200a-3p, miR-200b-3p, miR-215, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-382-5p, miR-325, miR-874

工作原理：

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template