細胞因子合成甲基化PCR芯片 Cytokine Production EpiTect Methyl qPCR Array
細胞因子合成甲基化PCR芯片 Cytokine Production EpiTect Methyl qPCR Array
細胞因子合成甲基化PCR芯片Cytokine Production EpiTect Methyl qPCR Array
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Cytokine Production EpiTect Methyl qPCR Array 細胞因子合成甲基化PCR芯片 The Human Cytokine Production EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes whose role in cytokine biosynthesis has been well documented. Profiling cellular or fresh tissue DNA samples with these arrays may help correlate CpG island methylation status with capacity of the resident immune or non-immune cells to produce and secrete cytokines.The results may also help provide insights into the molecular mechanisms and biological pathways behind immune disorders characterized by a lack or overabundance of cytokines in circulation. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different cytokine-producing genes with this DNA methylation PCR array. Both 96-well and 384-well ( 4 X 96 ) formats are available. The EpiTect Methyl II PCR Arrays use the MethylScreen? Technology provided under license from Orion Genomics, LLC 細胞因子合成甲基化PCR芯片用于研究文獻中已詳細報道其在細胞因子生物合成中的角色的22個基因的啟動子甲基化狀態(tài)。利用這些芯片分析細胞或新鮮組織基因組DNA樣本有助于關(guān)聯(lián)CpG島甲基化狀態(tài)與免疫或非免疫細胞產(chǎn)生和分泌細胞因子的能力。結(jié)果也可能幫助洞察以流通中細胞因子的缺乏或過多為特征的免疫紊亂的分子機制和生物學(xué)途徑。利用這個芯片，通過簡單的限制性內(nèi)切酶消化和實時定量PCR，就可以研究分析22個不同細胞因子合成基因的啟動子甲基化狀態(tài)。 T Cell Function Regulators: BCL10, BCL3, FOXP3, HMOX1, IL12A, MALT1, MAP3K7, SOD1, STAT5A, TRAF2, TRAF6. B Cell Function Regulators: BCL10, BCL3, INHA, INHBA, STAT5A. Transcriptional Regulators: BCL10, BCL3, FOXP3, GATA3, IRF1, SMAD3, STAT5A. Translational Regulators: BCL3, IGF2BP2. Environment & Intracellular Stimuli Responses: BCL10, BCL3, ELA2, FOXP3, GATA3, HMOX1, IL12A, INHA, INHBA, LTB, MALT1, MYD88, NOD1, SMAD3, SOD1, STAT5A, TLR2. Cytokine Production Signaling Molecules: BCL10, BCL3, FOXP3, HMOX1, INHA, INHBA, LTB, MALT1, MAP3K7, MYD88, NOD1, SMAD3, SOD1, STAT5A, TLR2, TRAF2, TRAF6 |
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