免疫毒性PCR芯片 Induced Pluripotent Stem Cells PCR Array
免疫毒性PCR芯片 Induced Pluripotent Stem Cells PCR Array
免疫毒性PCR芯片 Induced Pluripotent Stem Cells PCR Array
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| 簡(jiǎn)介： | Induced Pluripotent Stem Cells PCR Array 誘導(dǎo)多能干細(xì)胞PCR芯片 |
| 提供商： | SAbiosciences |
| 服務(wù)名稱： | 誘導(dǎo)多能干細(xì)胞PCR芯片 |
| 地區(qū)： | 美國(guó) |
“英拜為您實(shí)驗(yàn)加速” 技術(shù)服務(wù)網(wǎng)址：http://www.letsgrowganja.com/ 服務(wù)熱線：400-696-6643、 18019265738 郵箱：daihp@yingbio.com 、 huizhang1228@foxmail.com Induced Pluripotent Stem Cells PCR Array 誘導(dǎo)多能干細(xì)胞PCR芯片 he Human Induced Pluripotent Stem Cells RT2 Profiler PCR Array profiles the expression of 84 key genes involved in induced pluripotent stem cell (iPSC) research. iPSCs hold the promise to provide treatments for a multitude of diseases by converting adult somatic cells into pluripotent cells that are able to differentiate into any one of a variety of cell types, avoiding the ethics of embryonic stem cell (ESC) use. The process starts by transfecting or transducing somatic cells (such as keratinocytes) with constructs ectopically expressing a combination of specific transcription factors (KLF4, MYC, POU5F1, and/or SOX2). These transcription factors reprogram or induce the somatic cells to "dedifferentiate", losing markers of the original cell type and gaining markers of pluripotent cells. Often, a combination of additional transcription factors (such as ESRRB, LIN28A, NANOG, MYCN, and/or NR5A2) increases induction efficiency. To control the procedure, the expression of multiple gene classes included on this array must be monitored simultaneously: representative parental cell line genes, the ectopically expressed transcription factors, markers of iPSCs, and markers of the redifferentiation into ectoderm, endoderm, and mesoderm. ESCs and iPSCs have proven to not be functionally identical. Therefore, this array also analyzes genes highly expressed in both cell types to help distinguish them and better understand their differences. Because the expression of typical housekeeping or reference genes often proves inconsistent in these types of studies, the array includes another gene (NAT1) used in iPSC gene expression for data normalization if needed. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in the induced pluripotent stem cell dedifferentiation and redifferentiation processes with this array. The RT2 Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. 96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available. 誘導(dǎo)多能干細(xì)胞PCR芯片用于研究參與誘導(dǎo)多能干細(xì)胞(iPSC)的84個(gè)關(guān)鍵基因的表達(dá)。iPSCs有望治療大量疾病，通過(guò)轉(zhuǎn)化成年體細(xì)胞為多能細(xì)胞，使其分化成多種細(xì)胞類型中的任何一個(gè)，胚胎干細(xì)胞使用的倫理性。這個(gè)流程首先使轉(zhuǎn)染或轉(zhuǎn)導(dǎo)體細(xì)胞(如角化細(xì)胞)，構(gòu)建異位表達(dá)特定的轉(zhuǎn)錄因子的復(fù)合體(KLF4,MYC POU5F1,和/或SOX2)。這些轉(zhuǎn)錄因子重組或誘導(dǎo)體細(xì)胞“去分化”,失去原始細(xì)胞類型的標(biāo)記并獲得多能細(xì)胞的標(biāo)記。通常，額外的轉(zhuǎn)錄因子的結(jié)合(如ESRRB LIN28A,NANOG MYCN,和/或NR5A2)會(huì)增加感應(yīng)效率。為了控制該程序, 該芯片上的多種基因類群的表達(dá)必須同時(shí)被檢測(cè):代表親本的細(xì)胞系基因,異位表達(dá)的轉(zhuǎn)錄因子,iPSCs的標(biāo)記,再分化成外胚層、內(nèi)胚層和中胚層的標(biāo)記。ESCs和iPSCs已被證明功能不完全相同。因此,這個(gè)芯片分析兩者細(xì)胞類型都高表達(dá)的基因來(lái)幫助區(qū)分和更好的理解他們之間的差異。因?yàn)榈湫偷墓芗一騾⒖蓟虻谋磉_(dá)往往在這些類型的研究證明不一致,該芯片包含另一個(gè)基因(NAT1) 用于iPSC基因表達(dá)數(shù)據(jù)標(biāo)準(zhǔn)化。每張芯片含一個(gè)對(duì)照組使得分析數(shù)據(jù)時(shí)可以用相對(duì)定量ΔΔCT方法評(píng)估逆轉(zhuǎn)錄效率,基因組DNA污染,和PCR效率。利用這張芯片，通過(guò)實(shí)時(shí)定量PCR，可以簡(jiǎn)易且可靠地分析誘導(dǎo)多能干細(xì)胞去分化和再分化過(guò)程關(guān)鍵基因的表達(dá)。 PCR芯片僅用于分子生物學(xué)應(yīng)用。本產(chǎn)品不用于疾病的診斷、預(yù)防和治療。 提供96孔板——384 -(4 x 96)板,和100孔板 Somatic Cell Markers: Keratinocyte: CDH1, GJB2, KRT15, NUMB. Blood Stem Cell: CD34. Fibroblast: COL1A1, NCAM1. Neuronal Stem Cell: EMX2, FABP7, NES, OLIG2. Cardiomyocyte: PECAM1. Germ Cells: SYCP3. Induced Pluripotent Stem Cells: iPSC Markers: DNMT3B, PODXL, ZFP42. Other iPSC-Related Genes: ACTC1, ALDH1A1, ALDH2, APC, BGLAP, BMP2, BRIX1 (BXDC2), CCNE1, CD9, CDH2, COL2A1, COL9A1, EP300, FGF4, FGFR1, FOXD3, GABRB3, GJA1, GRB7, HDAC2, KAT2A, KAT7, KAT8, LEFTY1, LEFTY2, NODAL, PARD6A, REST, RUNX2, TERT. Reprogramming Factors: KLF4, MYC, POU5F1 (Oct3/4), SOX2. Other Reprogramming Factors: ESRRB, LIN28A, NANOG, MYCN, NR5A2. Reprogramming Enhancers & Inhibitors: AICDA, TP53, UTF1. Embryonic Stem Cells: ESC Markers: ALPL, GDF3, TDGF1 (CRIPTO), TERT. Other ESC-Related Genes: CCNA2, CDK1 (CDC2), CDC42, DPPA2, DPPA3, FGF2, HSPA9, MYBL2, OTX2, SOX15, TBX3, TCF3. Pluripotency Markers: ALPL, DNMT3B, FGF4, FOXD3, GDF3, LEFTY1, LEFTY2, NODAL, PODXL, UTF1, ZFP42. Early Differentiation Markers: Ectoderm: COL1A1, NCAM1, NES, PAX6, TUBB3. Mesoderm: CD34, GATA2, HAND1, MESP1, PECAM1, RUNX1. Endoderm: FOXA2, GATA4, HNF4A, SOX17. Housekeeping Gene: NAT1. |
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